1-5 December 2019
Birchwood
Africa/Johannesburg timezone
Note: Intel Keynote starts at 18:00 today (Monday)

Deciphering the “elixir of life”: Dynamic perspectives into the allosteric modulation of mitochondrial ATP synthase by J147, a novel drug in the treatment of Alzheimer’s disease

Not scheduled
5m
Birchwood

Birchwood

Poster SIG Seminar: Chemistry, Material Science and Health Science SIG Poster

Speaker

Mr Iwuchukwu Iwuchukwu Emmanuel (University of Kwazulu-Natal)

Description

The discovery of J147 represented a significant milestone in the treatment of age-related
disorders, which was further augmented by the recent identification of mitochondrial ATP
synthase as the therapeutic target. However, the underlying molecular events associated with
the modulatory activity of J147 has remained unresolved till date. Herein, we present, for the
first time, a dynamical approach to investigate the allosteric regulation of mATP synthase by
J147, using a reliable human αγβ protein model. The highlight of our findings is the existence
of the J147-bound protein in distinct structural associations at different MD simulation periods
coupled with concurrent open ↔ close transitions of the β catalytic and α allosteric (ATP5A)
sites as defined by Cα distances (d), TriCα (ϴ) and dihedral (ϕ) angular parameters. Firstly,
there was an initial pairing of the αγ subunits away from the β subunit followed by the
formation of the ‘non-catalytic’ αβ pair at a distance from the γ subunit. Interestingly, J147-
induced structural arrangements were accompanied by the systematic transition of the β
catalytic site from a closed to an open state while there was a concurrent transition of the
allosteric site from an open αE conformation to a closed state. Consequentially, J147 reduced
the structural activity of the whole αγβ complex while the unbound system exhibited high
atomistic deviations and structural flexibility. Furthermore, J147 exhibited favourable binding
at the allosteric site of mATP synthase with considerable electrostatic energy contributions
from Gln215, Gly217, Thr219, Asp312, Asp313, Glu371 and Arg406. These findings provide
details on the possible effects of J147 on mitochondrial bioenergetics, which could facilitate
the structure-based design of novel small-molecule modulators of mATP synthase in the
management of Alzheimer’s disease and other neurodegenerative disorders.

Primary authors

Mr Iwuchukwu Iwuchukwu Emmanuel (University of Kwazulu-Natal) Mr Agoni Clement (University of Kwazulu-Natal) Prof. Soliman Prof Mahmoud E.S. (University of Kwazulu-Natal)

Co-author

Dr Olotu Fisayo A. (University of Kwazulu-Natal)

Presentation Materials

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